Haemostasis, angiogenesis and vascular differentiation
Theme 1
The group focus on both fundamental and translational approaches to understand vessel pathophysiology in diseases. During the past years, D. Smadja has acquired expertise in the biology of Endothelial Progenitor Cells (EPC) in a fundamental and translational aspect. They are extremely rare and difficult to expand in vitro, in particular in patients with critical limb ischemia [1]. Their ontogeny as well as their implication in the formation of the vascular tree is controversial and not fully elucidated. The identification of the adult vascular stem cell remains open. Our research explores other sources of vasculogenic stem cells in bone marrow or tissues with differentiation potential (i.e. the embryonic-like stem cells VSEL) and the impact of the haemostasis pathway on the vascular compartment, to solve remaining therapeutic challenges such as the haemocompatibility of bioprosthetic materials and the treatment of ischemic diseases.
The mains projects are organised as follows:
- to identify new interactions of haemostasis with the angiogenic process,
- to analyse the functional vasculogenic capacities of Very small embryonic-like stem cells (VSELs),
- to evaluate the role of vasculogenic cells in the haemocompatibility of bioprosthetic valves and mechanical support,
- to understand the pathophysiology of arterial and venous thrombosis, exploring among other on the identification of the actors involved in arterial aging.
Part 1: New interactions of haemostasis with the angiogenic process:
Thrombus formation and wound healing are characterised by bidirectional interrelations between platelet / coagulation activation and angiogenesis. This project aims at studying the importance of haemostasis in its interaction with the angiogenic process and EPC differentiation. We have studied the involvement of Endoglin present on endothelial cells in normal and pathological conditions, in the intercellular adhesion of endothelial cells / EPC to mural cells and mesenchymal stem cells in revascularization. We now proposes to evaluate the importance of physical interaction of endoglin with cell membrane and explore the role of both membrane and soluble endoglin in the vasculogenesis process as well as in the haemostasis and arterial thrombosis.
Part 2: Very small embryonic-like stem cells (VSEL) as a source of vascular cells:
Because of their capacity to differentiate into endothelial cells and to promote post-ischemic revascularisation in a murine model of hindlimb ischemia [4], VSEL could represent a clinically relevant alternative to embryonic stem cells and induced pluripotent stem cells for cell therapy. Recently a group confirmed the endothelial differentiation of MSC derived from VSEL [5]. There are two major hurdles before these cells can be widely used in the clinic. First, they are very rare, and second, they reside in adult tissues in a quiescent state, due to erasure of methylation in regulatory sequences of parentally imprinted genes, a mechanism well known to maintain the quiescent state in primordial germ cells. Ratajczak’s group showed that it is now possible to expand VSEL in vitro when exposed to valproic acid and pituitary hormones [6]. We thus aim to explore their vasculogenic capacities and to investigate the possibility to use them as biomarkers of tissue regeneration.
Part 3 Valvular interstitial cells (VIC) and haemocompatibility of biomaterials:
The implantation of artificial valves or biomaterials can result in several complications including thrombosis.
The native aortic valve displays a layered architectural pattern of cells, including valvular endothelial cells (VEC) at the blood contacting surface as well as deep VIC. VIC participate to calcification process, have the ability to support EPC angiogenic properties and could be at the origin of both endothelial and perivascular lineage.
Aortic Valve Stenosis (AVS) can be treated by valve replacement using bioprosthetic material made with pericardial tissue from bovine origin. Such valves induce an acquired haemocompatibility and allow a lower dose of anticoagulant treatment to be given in patients, in contrast to mechanical valve [7]. However, after several years, these valves can become dysfunctional showing the same phenotype than the native one from AVS: fibrosis, neoangiogenic process, etc, recapitulating the classic history of native valve degradation but on a bioprosthetic material.
Therefore, our project aims:
- to better define the role of VIC in the occurrence of this dysfunction as well as to determine their ability to support angiogenesis in aging bioprosthetic valves,
- to study the haemocompatibility and complete endothelialisation of the cardiac bioprosthesis Carmat-total artificial heart and of extracorporeal CO2 purification technique.
Part 4: Pathophysiology of arterial and venous thrombosis:
Arterial thrombosis: Identification of the actors involved in arterial aging is a first essential stage in therapeutic approach. Firstly, by creating murine models of aging with a murine model characterized by the endothelial deletion of TXNIP (Thioredoxin-interacting protein), we were able to show that the Thioredoxin/TXNIP system was a major player in aging by protecting the artery from oxidative stress, inflammation and loss of aortic compliance induced by arterial aging. Moreover, using a model of ischemia of the lower limb, we observed that the endothelial deletion of TXNIP protected from the defect of post-ischemic revascularization during aging, confirming that endothelial TXNIP was a therapeutic target [8]. Our main project is to characterise the specific involvement of endothelial TXNIP and aging in our EC-KO TXNIP mice, to establish its involvement in angiogenic repair in vivo and ex vivo as well as to identify the actors involved with TRX-TXNIP system in this protection. We are also investigating whether the inhibition of TXNIP could be a strategy for the treatment of neuronal and vascular lesions after stroke.
Chronic venous thrombosis: O. Sanchez, B. Planquette and D. Smadja are partners of INNO-VTE that is a new F-CRIN-labelled network in France, aiming to develop translational clinical research and european-wide studies on venous thrombosis. Our expertise in chronic venous thrombo-embolism (VTE) led us to investigate the link between pulmonary vascular disease and the observed perfusion sequelae retained dyspnea frequently observed in patients with PE (Dyspnée 2 Study, O. Sanchez, clinical research initiation contract (CIRC)). We think that endothelial dysfunction is an important clinical and pathophysiological partner of sequelae in Pulmonary Embolism. Embolic sequelae can lead to Pulmonary Arterial Hypertension.
Theme 1 researchers
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PhD students
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Theme 1 team
Major publications 2020 / 2014
2020
[The role of direct oral anticoagulants in the management of cancer-associated thrombosis in 2020]. Auditeau C, Talbot A, Blandinières A, Smadja DM, Gendron N. Bull Cancer. 2020 May;107(5):574-585. doi: 10.1016/j.bulcan.2020.02.010.
SFBC working group “Biochemical markers of COVID-19”. Beauvieux MC, Bérard AM, Aimone-Gastin I, Barbé F, Barguil Y, Collin-Chavagnac D, Delacour H, Delevallée C, Nivet-Antoine V, Peoc’h K, Poupon C, Schmitt F, Piéroni L, Sapin V; Membres du Groupe de travail SFBC « Marqueurs biochimiques de COVID-19 »; Sous-groupe Privés; Sous-groupe Outre-Mer (OM)/francophonie; Sous-groupe CH; Sous-groupe Hôpitaux d’instruction des armées (HIA); Sous-groupe CHU Nord-Est; Sous-groupe CHU Ouest; Sous-groupe CHU Assistance publique-Hôpitaux de Paris (AP-HP); Sous-groupe CHU Sud; Sous-groupe CHU Auvergne Rhône-Alpes-Bourgogne Franche-Comté (ARA-BFC). Ann Biol Clin (Paris). 2020 Jun 1;78(3):269-277. doi: 10.1684/abc.2020.1563
Usefulness of immature platelet fraction measurement for diagnosis and monitoring of iron deficiency associated thrombocytopenia: about two cases. Blandinières A, Arlet JB, Gaussem P, Pouchot J, Darnige L. Ann Biol Clin (Paris). 2020 Aug 1;78(4):433-437. doi: 10.1684/abc.2020.1564.
Pulmonary embolism in patients with COVID-19 pneumonia. Bompard F, Monnier H, Saab I, Tordjman M, Abdoul H, Fournier L, Sanchez O, Lorut C, Chassagnon G, Revel MP.Eur Respir J. 2020 Jul 30;56(1):2001365. doi: 10.1183/13993003.01365-2020.
Is COVID-19 a New Hematologic Disease? Debuc B, Smadja DM. Stem Cell Rev Rep. 2020 May 12:1-5. doi: 10.1007/s12015-020-09987-4.
Usefulness of azacitidine therapy in a sickle cell disease patient with myelodysplastic syndrome. De Luna G, Darnige L, Roueff S, Peyrard T, Pouchot J, Arlet JB. Ann Hematol. 2020 Mar;99(3):661-662. doi: 10.1007/s00277-020-03940-8.
Respiratory mechanics and gas exchanges in the early course of COVID-19 ARDS: a hypothesis-generating study. Diehl JL, Peron N, Chocron R, Debuc B, Guerot E, Hauw-Berlemont C, Hermann B, Augy JL, Younan R, Novara A, Langlais J, Khider L, Gendron N, Goudot G, Fagon JF, Mirault T, Smadja DM. Ann Intensive Care. 2020 Jul 16;10(1):95. doi: 10.1186/s13613-020-00716-1.
Severity of endothelial dysfunction is associated with the occurrence of hemorrhagic complications in COPD patients treated by extracorporeal CO2 removal. Diehl JL, Augy JL, Rivet N, Guerin C, Chocron R, Smadja DM.Intensive Care Med. 2020 Jun 9. doi: 10.1007/s00134-020-06138-8.
Targeting endothelial thioredoxin-interacting protein (TXNIP) protects from metabolic disorder-related impairment of vascular function and post-ischemic revascularisation. Domingues A, Boisson-Vidal C, Marquet de Rouge P, Dizier B, Sadoine J, Mignon V, Vessières E, Henrion D, Escriou V, Bigey P, Chaussain C, Smadja DM, Nivet-Antoine V. Angiogenesis. 2020 May;23(2):249-264. doi: 10.1007/s10456-019-09704-x.
New Insights into the Use of Direct Oral Anticoagulants in Non-high Risk Thrombotic APS Patients: Literature Review and Subgroup Analysis from a Meta-analysis. Dufrost V, Darnige L, Reshetnyak T, Vorobyeva M, Jiang X, Yan XX, Gerotziafas G, Jing ZC, Elalamy I, Wahl D, Zuily S. Curr Rheumatol Rep. 2020 May 20;22(7):25. doi: 10.1007/s11926-020-00901-y.
Battle of the clones: paroxysmal nocturnal hemoglobinuria vs myelodysplastic syndrome. Friedrich C, Gay J, Alary AS, Arlet JB, Socie G, Fremaux-Bacchi V, Weiss IR, Kosmider O, Darnige L. Ann Hematol. 2020 Oct;99(10):2459-2461. doi: 10.1007/s00277-020-04134-y.
Effect of Early Initiation of Varenicline on Smoking Cessation in COPD Patients Admitted for Exacerbation: The Save Randomized Clinical Trial. Le Mao R, Tromeur C, Paleiron N, Sanchez O, Gagnadoux F, Jouneau S, Magnan A, Hayem-Vannimenus C, Dansou A, Proust A, Dion A, Larhantec G, Brestec AL, Dewitte JD, Roche N, Leroyer C, Couturaud F. COPD. 2020 Feb;17(1):7-14. doi: 10.1080/15412555.2019.1703928.
Clinical characteristics, management and outcome of COVID-19-associated immune thrombocytopenia: a French multicentre series. Mahévas M, Moulis G, Andres E, Riviere E, Garzaro M, Crickx E, Guillotin V, Malphettes M, Galicier L, Noel N, Darnige L, Terriou L, Guerveno C, Sanchis-Borja M, Moulinet T, Meunier B, Ebbo M, Michel M, Godeau B. Br J Haematol. 2020 Jul 17:10.1111/bjh.17024. doi: 10.1111/bjh.17024.
A Controlled Trial of Rivaroxaban after Transcatheter Aortic-Valve Replacement. Martin AC, Smadja DM, Karam N. N Engl J Med. 2020 Jul 9;383(2):e8. doi: 10.1056/NEJMc2017351.
Editorial: Recent Advances in Endothelial Progenitor Cells Toward Their Use in Clinical Translation. Medina RJ, Smadja DM. Front Med (Lausanne). 2020 Jan 8;6:290. doi: 10.3389/fmed.2019.00290.
Initial bridge to transplant experience with a bioprosthetic autoregulated artificial heart. Netuka I, Pya Y, Bekbossynova M, Ivak P, Konarik M, Gustafsson F, Smadja DM, Jansen P, Latrémouille C. J Heart Lung Transplant. 2020 Jul 14:S1053-2498(20)31655-7. doi: 10.1016/j.healun.2020.07.004.
Valproic Acid Decreases Endothelial Colony Forming Cells Differentiation and Induces Endothelial-to-Mesenchymal Transition-like Process. Nevo N, Lecourt S, Bièche I, Kucia M, Cras A, Blandinieres A, Vacher S, Gendron N, Guerin CL, Ratajczak MZ, Smadja DM. Stem Cell Rev Rep. 2020 Apr;16(2):357-368. doi: 10.1007/s12015-019-09950-y.
Evaluation of Venous Thromboembolism Recurrence Scores in an Unprovoked Pulmonary Embolism Population: A Post-hoc Analysis of the PADIS-PE trial. Raj L, Presles E, Le Mao R, Robin P, Sanchez O, Pernod G, Bertoletti L, Jego P, Lemarié CA, Leven F, Hoffmann C, Planquette B, Le Roux PY, Slaun PY, Nonent M, Girard P, Lacut K, Mélac S, Guégan M, Mismetti P, Laporte S, Meyer G, Leroyer C, Tromeur C, Couturaud F; PADIS-PE Investigators. Am J Med. 2020 Aug;133(8):e406-e421. doi: 10.1016/j.amjmed.2020.03.040.
Outpatient management of patients with low-risk pulmonary embolism: another piece of evidence. Sanchez O, Humbert M. Eur Heart J. 2020 Jan 21;41(4):519-521. doi: 10.1093/eurheartj/ehz484.
Angiopoietin-2 as a marker of endothelial activation is a good predictor factor for intensive care unit admission of COVID-19 patients. Smadja DM, Guerin CL, Chocron R, Yatim N, Boussier J, Gendron N, Khider L, Hadjadj J, Goudot G, Debuc B, Juvin P, Hauw-Berlemont C, Augy JL, Peron N, Messas E, Planquette B, Sanchez O, Charbit B, Gaussem P, Duffy D, Terrier B, Mirault T, Diehl JL.Angiogenesis. 2020 May 27:1-10. doi: 10.1007/s10456-020-09730-0.
Autoregulation of Pulsatile Bioprosthetic Total Artificial Heart is Involved in Endothelial Homeostasis Preservation. Smadja DM, Chocron R, Rossi E, Poitier B, Pya Y, Bekbossynova M, Peronino C, Rancic J, Roussel JC, Kindo M, Gendron N, Migliozzi L, Capel A, Perles JC, Gaussem P, Ivak P, Jansen P, Girard C, Carpentier A, Latremouille C, Guerin C, Netuka I. Thromb Haemost. 2020 Sep;120(9):1313-1322. doi: 10.1055/s-0040-1713751.
2019
In vivo efficacy of endothelial growth medium stimulated mesenchymal stem cells derived from patients with critical limb ischemia. Al-Rifai R, Nguyen P, Bouland N, Terryn C, Kanagaratnam L, Poitevin G, François C, Boisson-Vidal C, Sevestre MA, Tournois C. J Transl Med. 2019 Aug 9;17(1):261. doi: 10.1186/s12967-019-2003-3.
Treprostinil treatment decreases circulating platelet microvesicles and their procoagulant activity in pediatric pulmonary hypertension. Bacha NC, Levy M, Guerin CL, Le Bonniec B, Harroche A, Szezepanski I, Renard JM, Gaussem P, Israel-Biet D, Boulanger CM, Smadja DM.Pediatr Pulmonol. 2019 Jan;54(1):66-72. doi: 10.1002/ppul.24190.
Prognostic value of right ventricular dysfunction or elevated cardiac biomarkers in patients with low-risk pulmonary embolism: a systematic review and meta-analysis. Barco S, Mahmoudpour SH, Planquette B, Sanchez O, Konstantinides SV, Meyer G. Eur Heart J. 2019 Mar 14;40(11):902-910. doi: 10.1093/eurheartj/ehy873
Interleukin-8 release by endothelial colony-forming cells isolated from idiopathic pulmonary fibrosis patients might contribute to their pathogenicity. Blandinières A, Gendron N, Bacha N, Bièche I, Chocron R, Nunes H, Nevo N, Rossi E, Crestani B, Lecourt S, Chevret S, Lokajczyk A, Mignon V, Kisaoglu A, Juvin K, Bertil S, Valeyre D, Cras A, Gaussem P, Israël-Biet D, Smadja DM. Angiogenesis. 2019 May;22(2):325-339. doi: 10.1007/s10456-018-09659-5
Impact of 3-week citrulline supplementation on postprandial protein metabolism in malnourished older patients: The Ciproage randomized controlled trial. Bouillanne O, Melchior JC, Faure C, Paul M, Canouï-Poitrine F, Boirie Y, Chevenne D, Forasassi C, Guery E, Herbaud S, Le Corvoisier P, Neveux N, Nivet-Antoine V, Astier A, Raynaud-Simon A, Walrand S, Cynober L, Aussel C.Clin Nutr. 2019 Apr;38(2):564-574. doi: 10.1016/j.clnu.2018.02.017
Six months versus two years of oral anticoagulation after a first episode of unprovoked deep-vein thrombosis. The PADIS-DVT randomized clinical trial. Couturaud F, Pernod G, Presles E, Duhamel E, Jego P, Provost K, Pan-Petesch B, Sollier CBD, Tromeur C, Hoffmann C, Bressollette L, Lorillon P, Girard P, Le Moigne E, Le Hir A, Guégan M, Laporte S, Mismetti P, Lacut K, Bosson JL, Bertoletti L, Sanchez O, Meyer G, Leroyer C, Mottier D; “PADIS-DVT” investigators.Haematologica. 2019 Jul;104(7):1493-1501. doi: 10.3324/haematol.2018.210971.
In vitro and in vivo evaluation of a dextran-graft-polybutylmethacrylate copolymer coated on CoCr metallic stent. Delattre C, Velazquez D, Roques C, Pavon-Djavid G, Ollivier V, Lokajczyk A, Avramoglou T, Gueguen V, Louedec L, Caligiuri G, Jandrot-Perrus M, Boisson-Vidal C, Letourneur D, Meddahi-Pelle A. Bioimpacts. 2019;9(1):25-36. doi: 10.15171/bi.2019.04.
[Venous thromboembolism and urological cancer: Epidemiology and therapeutically management]. Felber M, Rozet F, Droupy S, Misraï V, Smadja DM, Rouprêt M.Prog Urol. 2019 Jan;29(1):1-11. doi: 10.1016/j.purol.2018.09.002.
Behavioral tests that reveal long-term deficits after permanent focal cerebral ischemia in mouse. Fréchou M, Margaill I, Marchand-Leroux C, Beray-Berthat V.Behav Brain Res. 2019 Mar 15;360:69-80. doi: 10.1016/j.bbr.2018.11.040.
Pain assessment and factors influencing pain during bone marrow aspiration: A prospective study. Gendron N, Zia Chahabi S, Poenou G, Rivet N, Belleville-Rolland T, Lemaire P, Escuret A, Ciaudo M, Curis E, Gaussem P, Siguret V, Darnige L. PLoS One. 2019 Aug 29;14(8):e0221534. doi: 10.1371/journal.pone.0221534
Potential usefulness of activated charcoal (DOAC remove®) for dRVVT testing in patients receiving Direct Oral AntiCoagulants. Jourdi G, Delrue M, Stepanian A, Valaize J, Foulon-Pinto G, Demagny J, Duchemin J, Nedelec-Gac F, Darnige L, Curis E, Delavenne X, Gaussem P, Siguret V, Gouin-Thibault I. Thromb Res. 2019 Dec;184:86-91. doi: 10.1016/j.thromres.2019.11.001.
Evaluation of the predictive value of the bleeding prediction score VTE-BLEED for recurrent venous thromboembolism. Klok FA, Presles E, Tromeur C, Barco S, Konstantinides SV, Sanchez O, Pernod G, Raj L, Robin P, Le Roux PY, Hoffman C, Mélac S, Bertoletti L, Girard P, Laporte S, Mismetti P, Meyer G, Leroyer C, Couturaud F; PADIS‐PE Investigators. Res Pract Thromb Haemost. 2019 May 25;3(3):364-371. doi: 10.1002/rth2.12214.
Cannabidiol Increases Proliferation, Migration, Tubulogenesis, and Integrity of Human Brain Endothelial Cells through TRPV2 Activation. Luo H, Rossi E, Saubamea B, Chasseigneaux S, Cochois V, Choublier N, Smirnova M, Glacial F, Perrière N, Bourdoulous S, Smadja DM, Menet MC, Couraud PO, Cisternino S, Declèves X. Mol Pharm. 2019 Mar 4;16(3):1312-1326. doi: 10.1021/acs.molpharmaceut.8b01252.
Ceramide 1-Phosphate Protects Endothelial Colony-Forming Cells From Apoptosis and Increases Vasculogenesis In Vitro and In Vivo. Mena HA, Zubiry PR, Dizier B, Mignon V, Parborell F, Schattner M, Boisson-Vidal C, Negrotto S. Arterioscler Thromb Vasc Biol. 2019 Oct;39(10):e219-e232. doi: 10.1161/ATVBAHA.119.312766.
Predictors for Residual Pulmonary Vascular Obstruction after Unprovoked Pulmonary Embolism: Implications for Clinical Practice-The PADIS-PE Trial. Raj L, Robin P, Le Mao R, Presles E, Tromeur C, Sanchez O, Pernod G, Bertoletti L, Jego P, Leven F, Lemarié CA, Martin A, Planquette B, Le Roux PY, Salaun PY, Nonent M, Girard P, Lacut K, Melac S, Mismetti P, Laporte S, Meyer G, Leroyer C, Couturaud F; PADIS-PE Investigators. Thromb Haemost. 2019 Sep;119(9):1489-1497. doi: 10.1055/s-0039-1692424.
Hemocompatibility and safety of the Carmat Total Artifical Heart hybrid membrane. Richez U, De Castilla H, Guerin CL, Gendron N, Luraghi G, Grimme M, Wu W, Taverna M, Jansen P, Latremouille C, Migliavacca F, Dubini G, Capel A, Carpentier A, Smadja DM. Heliyon. 2019 Dec 8;5(12):e02914. doi: 10.1016/j.heliyon.2019.e02914
Endoglin as an Adhesion Molecule in Mature and Progenitor Endothelial Cells: A Function Beyond TGF-β. Rossi E, Bernabeu C, Smadja DM. Front Med (Lausanne). 2019 Jan 30;6:10. doi: 10.3389/fmed.2019.00010.
Human Endothelial Colony Forming Cells Express Intracellular CD133 that Modulates their Vasculogenic Properties. Rossi E, Poirault-Chassac S, Bieche I, Chocron R, Schnitzler A, Lokajczyk A, Bourdoncle P, Dizier B, Bacha NC, Gendron N, Blandinieres A, Guerin CL, Gaussem P, Smadja DM. Stem Cell Rev Rep. 2019 Aug;15(4):590-600. doi: 10.1007/s12015-019-09881-8.
Outpatient Management of Patients With Pulmonary Embolism. Roy PM, Meyer G, Sanchez O, Huisman M. Ann Intern Med. 2019 Aug 6;171(3):227. doi: 10.7326/L19-0206.
[Recommendations of good practice for the management of thromboembolic venous disease in adults. Short version]. Sanchez O, Benhamou Y, Bertoletti L, Constant J, Couturaud F, Delluc A, Elias A, Fischer AM, Frappé P, Gendron N, Girard P, Godier A, Gut-Gobert C, Laporte S, Mahé I, Mauge L, Meneveau N, Meyer G, Mismetti P, Parent F, Pernod G, Quéré I, Revel MP, Roy PM, Salaün PY, Smadja DM, Sevestre MA. Rev Mal Respir. 2019 Feb;36(2):249-283. doi: 10.1016/j.rmr.2019.01.003.
Recipients with blood group A associated with longer survival rates in cardiac valvular bioprostheses. Schussler O, Lila N, Perneger T, Mootoosamy P, Grau J, Francois A, Smadja DM, Lecarpentier Y, Ruel M, Carpentier A. EBioMedicine. 2019 Apr;42:54-63. doi: 10.1016/j.ebiom.2019.02.047.
Vasculogenic Stem and Progenitor Cells in Human: Future Cell Therapy Product or Liquid Biopsy for Vascular Disease. Smadja DM. Adv Exp Med Biol. 2019;1201:215-237. doi: 10.1007/978-3-030-31206-0_11
Standardization of methods to quantify and culture endothelial colony-forming cells derived from peripheral blood: Position paper from the International Society on Thrombosis and Haemostasis SSC. Smadja DM, Melero-Martin JM, Eikenboom J, Bowman M, Sabatier F, Randi AM. J Thromb Haemost. 2019 Jul;17(7):1190-1194. doi: 10.1111/jth.14462.
[Recommendations for best practice in the management of venous thromboembolic disease in adults. Long version]. Sanchez O, Benhamou Y, Bertoletti L, Constans J, Couturaud F, Delluc A, Elias A, Fischer AM, Frappé P, Gendron N, Girard P, Godier A, Gut-Gobert C, Laporte S, Mahé I, Mauge L, Meneveau N, Meyer G, Mismetti P, Parent F, Pernod G, Quéré I, Revel MP, Roy PM, Salaün PY, Smadja DM, Sevestre MA. Rev Mal Respir. 2019 Jul 4:S0761-8425(19)30210-4. doi: 10.1016/j.rmr.2019.05.038.
Pregnancy-Adapted YEARS Algorithm for Diagnosis of Suspected Pulmonary Embolism. van der Pol LM, Tromeur C, Bistervels IM, Ni Ainle F, van Bemmel T, Bertoletti L, Couturaud F, van Dooren YPA, Elias A, Faber LM, Hofstee HMA, van der Hulle T, Kruip MJHA, Maignan M, Mairuhu ATA, Middeldorp S, Nijkeuter M, Roy PM, Sanchez O, Schmidt J, Ten Wolde M, Klok FA, Huisman MV; Artemis Study Investigators. N Engl J Med. 2019 Mar 21;380(12):1139-1149. doi: 10.1056/NEJMoa1813865.
2018
Bacha NC, Blandinieres A, Rossi E, Gendron N, Nevo N, Lecourt S, Guerin CL, Renard JM, Gaussem P, Angles-Cano E, Boulanger CM, Israel-Biet D, Smadja DM. Endothelial Microparticles are Associated to Pathogenesis of Idiopathic Pulmonary Fibrosis. Stem Cell Rev. 2018, Apr 14(2) :223-235. IF 3.1
Baldim V, Bedioui F, Mignet N, Margaill I, Berret JF. The enzyme-like catalytic activity of cerium oxide nanoparticles and its dependency on Ce3+ surface area concentration. Nanoscale. 2018 Apr 19;10(15):6971-6980.
Bedarida T, Domingues A, Baron S, Ferreira C, Vibert F, Cottart CH, Paul JL, Escriou V, Bigey P, Gaussem P, Leguillier T, Nivet-Antoine V. Reduced endothelial thioredoxin-interacting protein protects arteries from damage induced by metabolic stress in vivo. FASEB J. 2018 Jun;32(6):3108-3118.
Blandinières A, Gille T, Sadoine J, Bièche I, Slimani L, Dizier B, Gaussem P, Chaussain C, Planes C, Dorfmüller P, Israël-Biet D, Smadja DM. Endothelial Colony-Forming Cells Do Not Participate to Fibrogenesis in a Bleomycin-Induced Pulmonary Fibrosis Model in Nude Mice. Stem Cell Rev. 2018 Dec;14(6):812-822.
Boisson-Vidal C, Benslimane-Ahmim Z, Lokajczyk Z, Heymann D, Smadja DM. Osteoprotegerin induces CD34+ differentiation in endothelial progenitor cells. Front Med (Lausanne). 2018 Nov 27;5:331.
Alili R, Nivet-Antoine V, Saldmann A, Golmard JL, Cottart CH, Laguillier C, Giral P, Beaudeux JL, Bouillot JL, Poitou C, Clément K, Hébert-Schuster M. Human catalase gene promoter haplotype and cardiometabolic improvement after bariatric surgery. Gene. 2018 May 20;656:17-21. doi: 10.1016/j.gene.2018.02.064.
Bedarida T, Domingues A, Baron S, Ferreira C, Vibert F, Cottart CH, Paul JL, Escriou V, Bigey P, Gaussem P, Leguillier T, Nivet-Antoine V. Reduced endothelial thioredoxin-interacting protein protects arteries from damage induced by metabolic stress in vivo. FASEB J. 2018 Jun;32(6):3108-3118. doi: 10.1096/fj.201700856RRR.
d’Audigier C, Susen S, Blandinieres A, Mattot V, Saubamea B, Rossi E, Nevo N, Lecourt S, Guerin CL, Dizier B, Gendron N, Caetano B, Gaussem P, Soncin F, Smadja DM. Egfl7 Represses the Vasculogenic Potential of Human Endothelial Progenitor Cells. Stem Cell Rev. 2018 Feb;14(1):82-91. IF 3.1
El Amki M, Lerouet D, Garraud M, Teng F, Beray-Berthat V, Coqueran B, Barsacq B, Abbou C, Palmier B, Marchand-Leroux C, Margaill I. Improved Reperfusion and Vasculoprotection by the Poly(ADP-Ribose)Polymerase Inhibitor PJ34 After Stroke and Thrombolysis in Mice. Mol Neurobiol. 2018 Dec;55(12):9156-9168.
Goudot, G, Papadacci C, Dizier B, Baudrie V, Ferreira I, Boisson-Vidal C, Tanter M, Jeunemaitre X, Pernot M, Messas E, Mirault T. Arterial stiffening with ultrafast ultrasound imaging gives new insight into arterial phenotype of vascular Ehlers-Danlos mouse models. Accepté pour publication dans Ultraschall in der Medizin / European Journal of Ultrasound 2018 Sep 21. doi: 10.1055/a-0599-0841 IF 3,45
Latremouille C, Carpentier A, Leprince P, Roussel J-C, Cholley B, Boissier E, Epailly E, Capel A, Jansen P, Smadja DM https://pubmed-ncbi-nlm-nih-gov.proxy.insermbiblio.inist.fr/28986001-a-bioprosthetic-total-artificial-heart-for-end-stage-heart-failure-results-from-a-pilot-study/?from_term=smadja+DM&from_pos=9
Mena HA, Zubiry PR, Dizier B, Schattner M, Boisson-Vidal C, Negrotto S. Acidic preconditioning of endothelial colony-forming cells (ECFC) promote vasculogenesis under proinflammatory and high glucose conditions in vitro and in vivo. Stem Cell Res Ther. 2018 2018 May 2;9(1):120. doi: 10.1186/s13287-018-0872-7 IF 2.68
Rossi E, Pericacho M, Bachelot-Loza C, Pidard D, Gaussem P, Poirault-Chassac S, Blanco FJ, Langa C, González-Manchón C, Novoa JML, Smadja DM, Bernabeu C. Human endoglin as a potential new partner involved in platelet-endothelium interactions. Cell Mol Life Sci. 2018 Apr;75(7):1269-1284. IF 5.9
Schnell D, Planquette B, Berger A, Merceron S, Mayaux J, Strasbach L, Legriel S, Valade S, Darmon M, Meziani F. Cuff leak test for the diagnosis of post-extubation stridor. J Intensive Care Med. 2019 May;34(5):391-396.IF = 2,2
Teston E, Maldiney T, Marangon I, Volatron J, Lalatonne Y, Motte L, Boisson-Vidal C, Autret G, Clément O, Scherman D, Gazeau F, Richard C. Nanohybrids with Magnetic and Persistent Luminescence Properties for Cell Labeling, Tracking, In Vivo Real-Time Imaging, and Magnetic Vectorization. Small. 2018 Apr;14(16):e1800020. doi: 10.1002/smll.201800020. IF 8.64
Vincent F, Rauch A, Loobuyck V, Robin E, Nix C, Vincentelli A, Smadja DM, Leprince P, Amour J, Lemesle G, Spillemaeker H, Debry N, Latremouille C, Jansen P, Capel A, Moussa M, Rousse N, Schurtz G, Delhaye C, Paris C, Jeanpierre E, Dupont A, Corseaux D, Rosa M, Sottejeau Y, Barth S, Mourran C, Gomane V, Coisne A, Richardson M, Caron C, Preda C, Ung A, Carpentier A, Hubert T, Denis C, Staels B, Lenting PJ, Van Belle E, Susens S. …
Chapitre d’ouvrage
Boisson-Vidal C. Use of Marine Compounds to Treat Ischemic Diseases. in Blue Biotechnology: Production and Use of Marine Molecules First Edition. S. Bates, S. Labarre, Eds), Wiely-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, 2018, vol 1, chapitre 9, pp265–296.
2017
Benslimane-Ahmim Z, Pereira J, Lokajczyk A, Dizier B, Galy-Fauroux I, Fischer AM, Heymann D, Boisson-Vidal C. Osteoprotegerin regulates cancer cell migration through SDF-1/CXCR4 axis and promotes tumour development by increasing neovascularization. Cancer Lett. 2017;395:11-19. IF = 6,4
Bougouin W, Marijon E, Planquette B, Karam N, Dumas F, Celermajer DS, Jost D, Lamhaut L, Beganton F, Cariou A, Meyer G, Jouven X; on behalf from the Sudden Death Expertise Center. Pulmonary embolism related sudden cardiac arrest admitted alive at hospital: Management and outcomes. Resuscitation. 2017;115:135-140. IF = 5,2
Diehl JL, Chamoun C, Mercat A, Richard JC, Guérot E. An unusual sidestream capnogram. Br J Anaesth. 2017;118(5):807-808. IF = 6,3
Henao-Brasseur J, Bedel J, Mutlu G, Grimaldi D, Brasseur F, Laurent V, Troché G, Legriel S, Bedos JP, Planquette B. Transcutaneous CO2 monitoring: A new tool to identify spontaneous breathing trial failure during weaning from mechanical ventilation. A pilot cohort study. Intensive Care Med. 2016;42(6):78-79. IF = 12,0
Faivre L, Boucher H, Zerbib R, Domet T, Desproges A, Couzin C, Vanneaux V, Larghero J, Cras A. Cord blood-attached segment: is this a quality control to predict a good hematopoietic stem cell graft? Bone Marrow Transplant. 2017;doi: 10.1038/bmt.2017.150. IF = 3,9
Gille T, Didier M, Boubaya M, Sutton A, Carton Z, Baran-Marszak F, Sadoun-Danino D, Israël-Biet D, Cottin V, Gagnadoux F, Crestani B, Brillet PY, Valeyre D, Nunes H, Planès C. Obstructive sleep apnea and related comorbidities in incident idiopathic pulmonary fibrosis. Eur Respir J. 2017;49(6). pii: 1601934. IF = 10,6
Guérin CL, Blandinières A, Planquette B, Silvestre J-S, Israel-Biet D, Sanchez O, Smadja DM. Very small embryonic-like stem cells are mobilized in human peripheral blood during hypoxemic COPD exacerbations and pulmonary hypertension. Stem Cell Rev and Rep. 2017;13(4):561-566. IF = 3,1
Guérin CL, Rossi E, Saubamea B, Cras A, Mignon V, Silvestre JS, Smadja DM. Human very small embryonic-like cells support vascular maturation and therapeutic revascularization induced by endothelial progenitor cells. Stem Cell Rev and Rep. 2017; 13(4):552-560. IF = 3,1
Ribot J, Caliaperoumal G, Paquet J, Boisson-Vidal C, Petite H, Anagnostou F. Type 2 diabetes alters mesenchymal stem cell secretome composition and angiogenic properties. J Cell Mol Med. 2017;21(2):349-363. IF = 4,9
Rossi E, Goyard C, Cras A, Bacha N, Dizier B, Lokajczyk A, Planquette B, Mignon V, Bernabeu C, Sanchez O, Smadja DM. Co-injection of mesenchymal stem cells with endothelial progenitor cells accelerate muscle recovery in hind limb ischemia by an endoglin-dependent mechanism. Thromb Haemost. 2017;117(10) doi: 10.1160/TH17-01-0007. IF = 5,8
Smadja DM, Susen S, Rauch A, Cholley B, Latrémouille C, Duveau D, Zilberstein L, Méléard D, Boughenou MF, Van Belle E, Gaussem P, Capel A, Jansen P, Carpentier A. Carmat bioprosthetic total artificial heart is associated with an early hemostatic recovery and no acquired Willebrand syndrome in calves. J Cardiothor Vasc Anesthes. 2017; pii: S1053-0770(17)30246-X (sous presse) IF = 3,7
Smadja DM, Saubaméa B, Susen S, Kindo M, Van Belle E, Bruneval P, Jansen P, Roussel JC, Latrémouille C, Carpentier A. Bioprosthetic total artificial heart induces a profile of acquired hemocompatibility with membranes recellularization. J Am Coll Cardiol. 2017;18;70(3):404-406. IF = 19,8
Book chapter
Boisson-Vidal C. Use of Marine Compounds to Treat Ischemic Disease. In Blue Technology: Production and Use of Marine Molecules. Part II Marine Molecules for Disease Treatment / prevention and for Biological Research (S. Bates S. Labarre, Eds), Wiley VCH, Weinheim, Germany 2017 sous presse.
Smadja DM, Silvestre JS. Stem cell-based therapies for vascular regeneration in peripheral artery diseases. In Stem Cells in Toxicology and Medicine, Johns Wiley and Sons Ltd Ed, 2017, In press
2016
Bedarida T, Baron S, Vibert F, Ayer A, Henrion D, Thioulouse E, Marchiol C, Beaudeux JL, Cottart CH, Nivet-Antoine V. Resveratrol decreases TXNIP mRNA and protein nuclear expression with an arterial function improvement in old mice. J Gerontol A Biol Sci Med Sci. 2016;71:720-729. IF = 6,0
Diehl JL, Piquilloud L, Richard JM, Mancebo J, Mercat A. Effects of extracorporeal carbon dioxide removal on work of breathing in patients with chronic obstructive pulmonary disease. Intensive Care Med. 2016;42:951-952. IF = 12,1
Faisy C, Meziani F, Planquette B, Clavel M, Gacouin A, Bornstain C, Schneider F, Duguet A, Gibot S, Lerolle N, Ricard JD, Sanchez O, Djibre M, Ricome JL, Rabbat A, Heming N, Urien S, Esvan M, Katsahian S; for the DIABOLO investigators. Effect of acetazolamide vs placebo on duration of invasive mechanical ventilation among patients with chronic obstructive pulmonary disease. A randomized clinical trial. JAMA 2016;315(5):480-488. IF = 44,4
Oliveira MC, Labopin M, Henes J, Moore J, Papa ND, Cras A, Sakellari I, Schroers R, Scherer HU, Cuneo A, Kyrcz-Krzemien S, Daikeler T, Alexander T, Finke J, Badoglio M, Simões B, Snowden JA, Farge D. Does ex vivo CD34+ positive selection influence outcome after autologous hematopoietic stem cell transplantation in systemic sclerosis patients? Bone Marrow Transplant. 2016;51(4):501-505. IF = 3,6
Planquette B, Ferré A, Peron J, Vial-Dupuy A, Pastré J, Mourin G, Emmerich J, Collignon MA, Meyer G, Sanchez O. Residual pulmonary vascular obstruction and recurrence after acute pulmonary embolism. A single center cohort study. Thromb Res. 2016;148:70-75. IF = 2,7
Robin P, Le Roux PY, Planquette B, Accassat S, Roy PM, Couturaud F, Ghazzar N, Prevot-Bitot N, Couturier O, Delluc A, Sanchez O, Tardy B, Le Gal G, Salaun PY; MVTEP study group. Limited screening with versus without 18F-fluorodeoxyglucose PET/CT for occult malignancy is unprovoked venous thromboembolism: an open-label randomised controlled trial. Lancet Oncol. 2016;17(2):193-199. IF = 33,9
Rossi E, Smadja DM, Boscolo E, Langa C, Arevalo MA, Pericacho M, Gamella L, Kauskot A, Botella LM, Gaussem P, Bischoff J, Lopez-Novoa JM, Bernabeu C. Endoglin regulates mural cell adhesion in the circulatory system. Cell Mol Life Sci. 2016;73(8):1715-1739. IF = 5,9
2015
Rossi E, Smadja DM, Boscolo E, Langa C, Arevalo MA, Pericacho M, Gamella-Pozuelo L, Kauskot A, Botella LM, Gaussem P, Bischoff J, Lopez-Novoa JM, Bernabeu C. Endoglin regulates mural cell adhesion in the circulatory system. Cell Mol Life Sci. 2015 In press.
Bedarida T, Baron S, Vibert F, Ayer A, Henrion D, Thioulouse E, Marchiol C, Beaudeux JL, Cottart CH, Nivet-Antoine V. Resveratrol Decreases TXNIP mRNA and Protein Nuclear Expressions With an Arterial Function Improvement in Old Mice. J Gerontol A Biol Sci Med Sci. 2015 Jun 3.
Cottart CH, Nivet-Antoine V, Beaudeux JL. Is resveratrol an imposter? Mol Nutr Food Res. 2015 Jan;59(1):7.
d’Audigier C, Cochain C, Rossi E, Guérin CL, Bièche I, Blandinières A, Marsac B, Silvestre JS, Gaussem P, Smadja DM. Thrombin receptor PAR-1 activation on endothelial progenitor cells enhances chemotaxis-associated genes expression and leukocyte recruitment by a COX-2-dependent mechanism. Angiogenesis. 2015 Jul;18(3):347-59.
Guérin CL, Loyer X, Vilar J, Cras A, Mirault T, Gaussem P, Silvestre JS, Smadja DM. Bone-marrow-derived very small embryonic-like stem cells in patients with critical leg ischaemia: Evidence of vasculogenic potential. Thromb Haemost. 2015;113(5):1084-1094. IF = 5,3
Henno P, Maurey C, Le Pimpec-Barthes F, Devillier P, Delclaux C, Israël-Biet D. Is arginase a potential drug target in tobacco-induced pulmonary endothelial dysfunction? Respir Res. 2015 Mar 28;16:46.
Paquet J, Deschepper M, Moya A., Logeart-Avramoglou D, Boisson-Vidal C, Petite H. Oxygen tension regulates human mesenchymal stem cell (hMSC) paracrine functions. Stem Cells Transl Med. 2015 Jul;4(7):809-21.
Sapharikas E, Lokajczyk A, Fischer AM, Boisson-Vidal C. Fucoidan Stimulates Monocyte Migration via ERK/p38 Signaling Pathways and MMP9 Secretion. Mar Drugs. 2015 Jun 30;13(7):4156-4170
Segaliny A*, Mohamadi A*, Dizier B, Lokajczyk, Brion R, Charrier C, Boisson-Vidal C*, Heymann D*. IL-34 promotes tumour progression and metastatic process in osteosarcoma through induction of angiogenesis and macrophage recruitment. Int J Cancer. 2015 Jul 1;137(1):73-85
Smadja DM, Levy M, Huang L, Rossi E, Blandinières A, Israel-Biet D, Gaussem P, Bischoff J. Treprostinil indirectly regulates endothelial colony forming cell angiogenic properties by increasing VEGF-A produced by mesenchymal stem cells. Thromb Haemost. 2015 Sep 30;114(4):735-47.
Book chapter
Sun L, Cras A, Wang, Farge D. Cord Blood Cells and Autoimmune Diseases. In Cord Blood Stem Cells and Regenerative Medicine, Elsevier,Issy-les-Moulineaux, 2015.
2014
Guérin L, Couturaud F, Parent F, Revel M, Gillaizeau F, Planquette B, Pontal D, Guégan M, Gérald Simonneau G, Meyer G, Sanchez O. Prevalence of chronic thromboembolic pulmonary hypertension after acute pulmonary embolism. Thromb Haemost. 2014;112(3):598-605. IF = 5,6
Baron S, Bedarida T, Cottart CH, Vibert F, Vessieres E, Ayer A, Henrion D, Hommeril B, Paul JL, Renault G, Saubamea B, Beaudeux JL, Procaccio V, Nivet-Antoine V. Dual effects of resveratrol on arterial damage induced by insulin resistance in aged mice. J Gerontol A Biol Sci Med Sci. 2014 Mar;69(3):260-9.
Bedarida T, Baron S, Vessieres E, Vibert F, Ayer A, Marchiol-Fournigault C, Henrion D, Paul JL, Noble F, Golmard JL, Beaudeux JL, Cottart CH, Nivet-Antoine V. High-protein-low-carbohydrate diet: deleterious metabolic and cardiovascular effects depend on age. Am J Physiol Heart Circ Physiol. 2014 Sep 1;307(5):H649-57.
Cottart CH, Nivet-Antoine V, Beaudeux JL. Review of recent data on the metabolism, biological effects, and toxicity of resveratrol in humans. Mol Nutr Food Res. 2014 Jan;58(1):7-21.
d’Audigier C, Gautier B, Yon A, Alili JM, Guérin CL, Evrard SM, Godier A, Haviari S, Reille-Serroussi M, Huguenot F, Dizier B, Inguimbert N, Borgel D, Bièche I, Boisson-Vidal C, Roncal C, Carmeliet P, Vidal M, Gaussem P, Smadja DM. Targeting VEGFR1 on endothelial progenitors modulates their differentiation potential. Angiogenesis. 2014 Jul;17(3):603-16.
Mauge L, Sabatier F, Boutouyrie P, D’Audigier C, Peyrard S, Bozec E, Blanchard A, Azizi M, Dizier B, Dignat-George F, Gaussem P, Smadja DM. Forearm ischemia decreases endothelial colony-forming cell angiogenic potential. Cytotherapy. 2014 Feb;16(2):213-24.
Mena HA, Boisson-Vidal C, Lokajczyk A, Dizier B, Strier SE, Voto LS, Schattner M, Negrotto S. Acidic preconditoning improves the proangiogenic responses of endothelial colony forming cells. Angiogenesis. 2014 Oct;17(4):867-79
Mezzapesa A, Orset C, Plawinski L, Doeuvre L, Martinez de Lizarrondo S, Chimienti G, Vivien D, Mansour A, Matà S, Pepe G, Anglés-Cano E. Plasminogen in cerebrospinal fluid originates from circulating blood. J Neuroinflammation. 2014 Sep 16;11:154.
Smadja DM, Dorfmüller P, Guerin CL, Bieche I, Badoual C, Boscolo E, Kambouchner M, Cazes A, Mercier O, Humbert M, Gaussem P, Bischoff J, Israël-Biet D. Cooperation between human fibrocytes and endothelial colony-forming cells increases angiogenesis via the CXCR4 pathway. Thromb Haemost. 2014 Nov;112(5):1002-13.
Smadja DM, Nunes H, Juvin K, Bertil S, Valeyre D, Gaussem P, Israel-Biet D. Increase in both angiogenic and angiostatic mediators in patients with idiopathic pulmonary fibrosis. Pathol Biol (Paris). 2014 Dec;62(6):391-4.
Smadja DM. Bone marrow cell therapy in cardiovascular disease drives us slowly to a better identification of the active cell component. Stem Cell Res Ther. 2014 Jan 27;5(1):16.
Smadja DM. Neutrophils as new conductors of vascular homeostasis. J Thromb Haemost. 2014 Jul;12(7):1166-9.
Book chapter
Smadja DM, Lévy BI, Silvestre JS. Endothelial progenitor cells and cardiovascular ischemic diseases: characterization, functions and potential clinical applications as biomarker and therapeutic tools. In Molecular Mechanisms of Angiogenesis From Ontogenesis to Oncogenesis, Springer Ed, Paris, 2014.